Cousins Bo (Seann William Scott), Luke (Johnny Knoxville), and Daisy Duke (Jessica Simpson) run a moonshine business for their Uncle Jesse (Willie Nelson) in Hazzard County, Georgia in modern day. Hazzard is a small town where everybody knows everyone else. The town moves very slowly, except the Dukes. The cousins' primary mode of transportation is an orange 1969 Dodge Charger that the boys affectionately refer to as 'The General Lee'. Along the way, the family is tormented by corrupt Hazzard County Commissioner Jefferson Davis Hogg, widely known as "Boss Hogg" (Burt Reynolds), Sheriff Rosco P. Coltrane (M.C. Gainey), and his willing but dimwitted henchman Deputy Enos Strate (Michael Weston). Bo and Luke Duke are on a moonshine delivery when one of their customers finds Luke having sex with his sister. The brother and his father chase the Duke brothers but Bo gets The General Lee stuck at a construction site on a farm. Bo drove over a broken bridge and avoids an oncoming truck breaking a fence and getting inside a farm where he gets the car stuck between 2 huge construction machines. Billy Prickett (James Roday), a famous stock-car driver, enters Hazzard to participate in the annual Hazzard County Rally having won four times before becoming a professional racer, a record Bo seeks to break. A fight breaks out between the Dukes and Prickett's crew after one of the Prickett's makes a move on Daisy. The fight gets out of hand and is stopped by Coltrane, who tells the Dukes the construction workers vandalized their car, and it was towed to a local mechanic, Cooter Davenport. This would ruin Bo's attempt at winning his fifth consecutive local Hazzard Road rally, as the car is not likely to be back in shape for the weekend for the rally. Cooter shows the wrecked car to the Dukes and says that he will try to get it running again, but there is no way that it will race in the Hazzard Rally. Meanwhile, Coltrane plants a fake moonshine still ("because he's too dumb to find the real one") in Uncle Jesse's barn and seizes the Duke property in the interest of eminent domain for Boss Hogg. Jesse wanted to fight Rosco, but Big Hoss came in with reinforcements, thereby forcing the family to temporarily reside with the neighbor and Uncle Jesse's love-interest, Pauline (Lynda Carter). Pauline informs the Dukes that Coltrane seized another farm on similar charges, Bo and Luke investigate a local construction site and find their farm and neighboring farms marked on a map. They also discover a safe which they believe contains information that Boss Hogg wants to keep secret. Unable to get the safe open, they drag it to Sheev's (Kevin Heffernan). With his help they blow open the safe and discover geologic core samples but are unsure on what the samples are. Meanwhile, Coltrane makes arrangements to seize the General Lee as "evidence" from the local auto body shop run by the Dukes' friend Cooter (David Koechner). Having learned Rosco's plans to impound and auction off the General Lee, Cooter calls his friends to repair and soup up the General Lee. Cooter turns the car into a hot-rod and applies a new paint job and horn, in return for finally getting payment for all the work he has done ("...because that's how this works.") for the boys in the past, when Bo wins the Rally. After retrieving the General Lee before Rosco can, the Dukes go to Atlanta to visit a local university geology lab, meeting up with Katie-Lynn Johnson (Nikki Griffin), a Hazzard County girl and the Dukes' love interest, and her roommate Annette (Jacqui Maxwell). At the lab, they discover Boss Hogg's intentions of turning the county into a strip coalmine. They are later arrested after running from campus police. Back in Hazzard, Daisy learns, with the help of Sheriff Deputy Enos Strate (Michael Weston), that Billy Prickett has been hired by Boss Hogg to participate in the Rally as a ringer. Boss Hogg then heads to Atlanta where he informs the Duke boys, in lock-up, that they are too late to stop him and reveals that the vote on Hogg's proposition is at the same time as the rally, explaining Billy Prickett's involvement. During a transfer from detainment, Daisy helps the boys escape, and they speed home to try to inform the townsfolk, escaping the Atlanta Police, and the Georgia State Patrol. Upon returning home, the Dukes discover that Coltrane has taken Uncle Jesse and Pauline hostage, an obvious trap for the boys, and that Billy is in on the scheme because he's ashamed of the town's low status. The two race to the farmhouse to cause a distraction while Daisy and Cooter rescue Jesse and Pauline. Meanwhile, the college girls head to the rally with Sheev to inform the townsfolk about the vote on the strip-mining ordinance. Because of Sheev's armadillo hat and lack of pants, and the fact that Sheev is a known conspiracy theorist, no one listens to him. So, Bo leaves for the rally while Luke and Jesse team up to foil the police who are chasing Bo, interfering with the race. Upon crossing the finish line first, before Billy, the two continue racing back and forth all the way into town, leading the townsfolk to the courthouse just in time to vote against Hogg's proposed ordinance. At the courthouse, Daisy takes advantage of the governor of Georgia's presence and TV cameras to convince him into pardoning the boys, so Uncle Jesse takes the opportunity to knock out Boss Hogg and gets a pardon for assaulting a county commissioner at the same time. The film ends with a cook-out at the Dukes' house where Pauline convinces Uncle Jesse, who could not be found because he was "using the meat smoker," to get up and play the television series' main theme. Bo and Luke are romantically involved with the girls in General Lee when they are caught by Luke's other love-interest Laurie Pullman (Alice Greczyn) from the intro of the film, who proceeds to chase them with a shotgun as they drive away.
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